The subcellular protein atlas displays high resolution, multicolor images of immunofluorescently stained cells. This provides spatial information on protein expression patterns on a fine cellular and subcellular level. For each antibody two human suitable cell lines from a cell line panel are selected for the immunofluorescence analysis, based on RNA sequencing data. U-2 OS is also always used, making up the third cell line. To validate the results, the corresponding target protein may be downregulated by siRNA prior to immunostaining.
Example proteins in the subcell atlas:
Ezrin
EZR, VIL2.
Protein localized to the plasma membrane in both human and mouse cells.
ZFPL1
Zinc finger protein-like 1.
Protein localized to the Golgi apparatus.
HNRNPUL1
Heterogeneous nuclear ribonucleoprotein U-like 1.
Localized to the nucleus but excluded from the nucleoli in human and mouse cells. The location has been validated by siRNA mediated gene silencing.
The search function can be used for free text search (type anything in the search field), or for more complex queries using "Fields" (see examples).
The free text search will scan for complete and partial matches to gene names, gene synonyms, gene descriptions, external (UniProt, Ensembl, NCBI Entrez Gene) gene and protein identifiers, protein classes, Gene Ontology identifiers and descriptions, antibody identifiers and image annotations. The free text query word needs to be at least three characters long.
