ACSS3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039353

 

Antibody HPA061517

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA039353 HPA061517 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

92 98 

Antigen sequence

EYKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENKHSPSTRWFVEGML
NICYNAVDRHIENGKGDKIAIIYDSPVTNTKATFTYKEVLEQ
 
MDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDP
LKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVK
 

Matching transcripts

ACSS3-001 - ENSP00000449535 [100%]
ACSS3-002 - ENSP00000261206 [99%]
 ACSS3-001 - ENSP00000449535 [100%]
ACSS3-002 - ENSP00000261206 [100%]
ACSS3-003 - ENSP00000448965 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows STRONG CYTOPLASMIC positivity in hepatocytes.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:125  

Literature conformity

No avaliable characterization data  

RNA consistency

Not done  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

74.8, 74.6 74.8, 74.6, 40.3 

Antibody dilution

1:250 1:890 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:10600 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 

Antibody dilution

 1:200 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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ACSS3-001
 
ACSS3-002
 
ACSS3-003