OTX1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA052122

 

Antibody CAB012341

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 

Product name

HPA052122 ab25985 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

11 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

66  

Antigen sequence

TAAASYPMSYGQGGSYGQGYPTPSSSYFGGVDCSSYLAPMHSHHHPHQLS
PMAPSSMAGHHHHHPH
 
 

Matching transcripts

OTX1-001 - ENSP00000282549 [100%]
OTX1-002 - ENSP00000355631 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:6 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.3 37.3, 10.7 

Antibody dilution

1:80 1:500 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected 

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:75  

Literature conformity

Consistent with extensive gene/protein characterization data  

RNA consistency

Mainly consistent with RNAseq data  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:550  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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OTX1-001
 
OTX1-002