PLCD4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038139

 

Antibody HPA038140

 

Antibody CAB009914

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA038139 HPA038140 sc-30063 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

13 13 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein 

Length (aa)

78 75  

Antigen sequence

MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHA
RQARGSAKPSFSISDVETIRNGHDSELL
 
GFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWL
SDWFQRGDKNQDGKMSFQEVQRLLH
 
 

Matching transcripts

PLCD4-001 - ENSP00000388631 [100%]
PLCD4-006 - ENSP00000396942 [100%]
PLCD4-007 - ENSP00000396185 [100%]
PLCD4-011 - ENSP00000391850 [100%]
 PLCD4-001 - ENSP00000388631 [100%]
PLCD4-006 - ENSP00000396942 [100%]
PLCD4-007 - ENSP00000396185 [100%]
  

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
More information
 Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:150 1:2000 1:150 

Literature conformity

Consistent with gene/protein characterization data Consistent with gene/protein characterization data Consistent with gene/protein characterization data 

RNA consistency

Consistent with RNAseq data Mainly consistent with RNAseq data Consistent with RNAseq data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

91, 87.6, 11.5 91, 87.6 91, 87.6, 13.9, 12.9, 11.5, 7.8 

Antibody dilution

1:250 1:250 1:500 

Validation WB

Uncertain: No bands detected Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:30000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line HeLa shows positivity in plasma membrane & cytoplasm.
More information
 Application not done for this antibody. 

Antibody dilution

 1:200  

Validation IF

 Supportive: The subcellular location is supported by literature.  
 

ANTIGEN VIEW

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PLCD4-001
 
PLCD4-006
 
PLCD4-007
 
PLCD4-011