USP35

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA055213

 

Antibody HPA058592

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA055213 HPA058592 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

79 59 

Antigen sequence

PPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITED
TPPTSLYIEGLDSKEAGGQSSQEERIERE
 
LYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGN
GGDFHRLVF
 

Matching transcripts

USP35-001 - ENSP00000431876 [100%]
USP35-004 - ENSP00000435468 [100%]
USP35-007 - ENSP00000434942 [100%]
USP35-201 - ENSP00000400825 [100%]
 USP35-001 - ENSP00000431876 [100%]
USP35-004 - ENSP00000435468 [100%]
USP35-007 - ENSP00000434942 [100%]
USP35-201 - ENSP00000400825 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human skeletal muscle shows nucelar and cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:900 1:120 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line HeLa shows positivity in nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:200  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

113.4, 84.2, 68, 65.7 113.4, 84.2, 68, 65.7 

Antibody dilution

1:1750 1:180 

Validation WB

Uncertain: No bands detected Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:18000 1:2200 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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USP35-001
 
USP35-004
 
USP35-007
 
USP35-201