DNAJC8

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026275

 

Antibody HPA026283

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026275 HPA026283 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

13 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

84 77 

Antigen sequence

EGSRGGWAGEMAASGESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSK
NQIERLTRPGSSYFNLNPFEVLQIDPEVTDEEIK
 
ELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVD
SWRNFQANTKGKKEKKNRTFLRPPKVK
 

Matching transcripts

DNAJC8-001 - ENSP00000263697 [88%]
 DNAJC8-001 - ENSP00000263697 [100%]
DNAJC8-006 - ENSP00000474440 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line HeLa shows positivity in cytoplasm, cytokinetic bridge & nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:23  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         siRNA

Image

  

Description

Signal downregulation > 25% by one siRNA.
More information
 Application not done for this antibody. 

Antibody dilution

1:23  

Validation siRNA

Supportive: The siRNA validation is supportive.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

29.8 29.8, 13 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:65 

Literature conformity

 No avaliable characterization data 

RNA consistency

 Mainly consistent with RNAseq data 
 

ANTIGEN VIEW

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DNAJC8-001
 
DNAJC8-006