FHAD1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA054284

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA054284 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

82 

Antigen sequence

LQEMGNRESVIKINLERAVGQLEHFRSQVIKATYGRAKPFRDKPVTDQQL
IEKITQVTEDNINFQQKKWTLQKETQLSNSKQ
 

Matching transcripts

FHAD1-026 - ENSP00000365166 [100%]
FHAD1-202 - ENSP00000351770 [100%]
FHAD1-203 - ENSP00000365167 [100%]
FHAD1-205 - ENSP00000407615 [100%]
FHAD1-011 - ENSP00000365165 [82%]
FHAD1-204 - ENSP00000383868 [82%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:25 

Literature conformity

No avaliable characterization data 

RNA consistency

Mainly consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

166.3, 161.9, 157.7, 37.3, 36.4 

Antibody dilution

1:110 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:900 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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FHAD1-011
 
FHAD1-026
 
FHAD1-202
 
FHAD1-203
 
FHAD1-204
 
FHAD1-205