TBC1D7

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA034748

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA034748 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

62 

Antigen sequence

TRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPKLPYD
LWFKRCFAGCLP
 

Matching transcripts

TBC1D7-003 - ENSP00000412102 [100%]
TBC1D7-004 - ENSP00000394425 [100%]
TBC1D7-005 - ENSP00000414292 [100%]
TBC1D7-006 - ENSP00000417005 [100%]
TBC1D7-008 - ENSP00000348813 [100%]
TBC1D7-009 - ENSP00000368602 [100%]
TBC1D7-010 - ENSP00000404680 [100%]
TBC1D7-012 - ENSP00000475727 [100%]
TBC1D7-020 - ENSP00000475191 [100%]
TBC1D7-202 - ENSP00000368609 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:200 

Literature conformity

No avaliable characterization data 

RNA consistency

Not done 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

34, 30.7, 29.2, 27.7, 24.2, 23.3, 22.8 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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TBC1D7-003
 
TBC1D7-004
 
TBC1D7-005
 
TBC1D7-006
 
TBC1D7-008
 
TBC1D7-009
 
TBC1D7-010
 
TBC1D7-012
 
TBC1D7-020
 
TBC1D7-202