LRRIQ3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA030798

 

Antibody HPA030799

 

Antibody HPA030801

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA030798 HPA030799 HPA030801 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

79 82 84 

Antigen sequence

QIKSLPNTKFWNGLKNLKLLYLHDNGFAKLKNICVLSACPTLIALTMFDC
PVSLKKGYRHVLVNSIWPLKALDHHVISD
 
MFHGTVTEELTSHEEWSHYNENIREGQKDFVFVKFNGLHLKSMENLQSCI
SLRVCIFSNNFITDIHPLQSCIKLIKLDLHGN
 
QRAGMKLRTFSDIDKYYTEQKKQEYHKEKVRVVAMAQVARERVRVAVNEH
LNQKKYATQKLIEENKETIQNSLRQVWQNRFNYL
 

Matching transcripts

LRRIQ3-001 - ENSP00000359948 [100%]
LRRIQ3-003 - ENSP00000415319 [100%]
LRRIQ3-008 - ENSP00000378524 [100%]
LRRIQ3-201 - ENSP00000346414 [100%]
 LRRIQ3-001 - ENSP00000359948 [100%]
LRRIQ3-003 - ENSP00000415319 [100%]
LRRIQ3-004 - ENSP00000359946 [100%]
LRRIQ3-008 - ENSP00000378524 [100%]
LRRIQ3-201 - ENSP00000346414 [100%]
 LRRIQ3-008 - ENSP00000378524 [100%]
LRRIQ3-201 - ENSP00000346414 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
More information
 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:125 1:450 1:325 

Literature conformity

No avaliable characterization data No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done Not done 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

73.7, 35.3, 23.2 73.7, 35.3, 23.2, 22.7 73.7 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane.
More information
 Application not done for this antibody. 

Antibody dilution

 1:30  

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available.  
 

ANTIGEN VIEW

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LRRIQ3-001
 
LRRIQ3-003
 
LRRIQ3-004
 
LRRIQ3-008
 
LRRIQ3-201