GSTA4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA048934

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA048934 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

25 

Antigen sequence

FQQVPMVEIDGMKLVQTRAILNYIA
 

Matching transcripts

GSTA4-001 - ENSP00000360002 [92%]
GSTA4-003 - ENSP00000359998 [92%]
 

Other gene match

GSTA3 - ENSG00000174156 [100%]
GSTA5 - ENSG00000182793 [100%]
GSTA1 - ENSG00000243955 [100%]
GSTA2 - ENSG00000244067 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:2000 

Literature conformity

Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly not consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

25.7, 25.6, 25.3, 19.7, 12.5 

Antibody dilution

1:890 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:7100 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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GSTA4-001
 
GSTA4-003