RAB40C

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA055931

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA055931 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

41 

Antigen sequence

SMANGMNAVMMHGRSYSLASGAGGGGSKGNSLKRSKSIRPP
 

Matching transcripts

RAB40C-001 - ENSP00000248139 [100%]
RAB40C-003 - ENSP00000455225 [100%]
RAB40C-201 - ENSP00000438492 [100%]
RAB40C-202 - ENSP00000438382 [100%]
RAB40C-203 - ENSP00000445050 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:300 

Literature conformity

Not consistent with gene/protein characterization data 

RNA consistency

Not at all consistent with RNAseq data 

         Western Blot

Image

 

Description

 

Target mass (kDa)

31.3, 26.4 

Antibody dilution

1:150 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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RAB40C-001
 
RAB40C-003
 
RAB40C-201
 
RAB40C-202
 
RAB40C-203