TSSC1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA031231

 

Antibody HPA031232

 

Antibody HPA031233

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA031231 HPA031232 HPA031233 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 6 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

88 91 82 

Antigen sequence

VILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEH
EDSVYAVDWSSADPWLFASLSYDGRLVINRVPRALKYH
 
SKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMAC
VVWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLE
 
IYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINK
NVLLHQAGEIWHISASPADRGVLTTCYNRTSD
 

Matching transcripts

TSSC1-001 - ENSP00000371559 [100%]
TSSC1-003 - ENSP00000381652 [100%]
 TSSC1-001 - ENSP00000371559 [100%]
TSSC1-003 - ENSP00000381652 [100%]
TSSC1-004 - ENSP00000389080 [93%]
 TSSC1-001 - ENSP00000371559 [100%]
TSSC1-004 - ENSP00000389080 [100%]
TSSC1-003 - ENSP00000381652 [98%]
TSSC1-009 - ENSP00000403043 [98%]
TSSC1-010 - ENSP00000400214 [96%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:1000 1:150 1:25 

Literature conformity

No avaliable characterization data No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done Not done 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418772)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418772)
More information
 

Target mass (kDa)

46.3, 43.6 46.3, 43.6, 34.9 46.3, 43.6, 34.9, 17.2, 15.1 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm, centrosome & nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

 1:75  

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available.  
 

ANTIGEN VIEW

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TSSC1-001
 
TSSC1-003
 
TSSC1-004
 
TSSC1-009
 
TSSC1-010