PTPRZ1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA015103

 

Antibody CAB025106

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 R&D Systems
 

Product name

HPA015103 MAB2688 

Host species

Rabbit Rat 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

4 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein 

Length (aa)

148  

Antigen sequence

LFRHLHTVSQILPQVTSATESDKVPLHASLPVAGGDLLLEPSLAQYSDVL
STTHAASETLEFGSESGVLYKTLMFSQVEPPSSDAMMHARSSGPEPSYAL
SDNEGSQHIFTVSYSSAIPVHDSVGVTYQGSLFSGPSHIPIPKSSLIT
 
 

Matching transcripts

PTPRZ1-001 - ENSP00000377047 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
More information
 Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:1200 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data Mainly consistent with RNAseq data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
More information
 Application not done for this antibody. 

Antibody dilution

1:61  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Target too small/large to be analyzed with the present setup Target too small/large to be analyzed with the present setup 

Target mass (kDa)

254.6 254.6, 162.7 

Antibody dilution

  

Validation WB

Target too small/large to be analyzed with the present setup Target too small/large to be analyzed with the present setup 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:6000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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PTPRZ1-001