CD68

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA048982

 

Antibody CAB000051

 

Antibody CAB000066

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 DakoCytomation
 DakoCytomation
 

Product name

HPA048982 M0814 M0876 

Host species

Rabbit Mouse Mouse 

Clonality

pAb mAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Supernatant Supernatant 

Released in version

11 1 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Native protein Native protein 

Length (aa)

72   

Antigen sequence

SPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPN
KTKVQGSCEGAHPHLLLSFPYG
 
 
 

Matching transcripts

CD68-001 - ENSP00000250092 [100%]
CD68-002 - ENSP00000369867 [100%]
   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
More information
 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells.
More information
 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in Kupffer cells.
More information
 

Retrieval method

HIER pH6 HIER pH9 HIER pH9 

Antibody dilution

1:4000 1:15000 1:200 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done Not done 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in the Golgi apparatus & vesicles.
More information
 Application not done for this antibody. 

Antibody dilution

 1:400  

Validation IF

 Supportive: The subcellular location is supported by literature.  

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.4, 34.7 37.4, 34.7, 18.6, 13.2 37.4, 34.7, 18.6, 13.2 

Antibody dilution

1:350 1:500 1:500 

Validation WB

Uncertain: No bands detected Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Uncertain: No bands detected 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:2800   

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).   
 

ANTIGEN VIEW

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CD68-001
 
CD68-002