NASP

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA028136

 

Antibody HPA030518

 

Antibody HPA030520

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA028136 HPA030518 HPA030520 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 12 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

78 96 96 

Antigen sequence

DVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEA
FFFYGKSLLELARMENGVLGNALEGVHV
 
KESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSS
NCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSG
 
GTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVE
AESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAE
 

Matching transcripts

NASP-001 - ENSP00000255120 [100%]
NASP-005 - ENSP00000255121 [100%]
NASP-007 - ENSP00000400792 [100%]
NASP-015 - ENSP00000436939 [100%]
NASP-201 - ENSP00000384529 [100%]
 NASP-001 - ENSP00000255120 [100%]
NASP-004 - ENSP00000361122 [100%]
NASP-005 - ENSP00000255121 [100%]
NASP-201 - ENSP00000384529 [100%]
NASP-202 - ENSP00000438871 [100%]
 NASP-001 - ENSP00000255120 [100%]
NASP-009 - ENSP00000436924 [100%]
NASP-201 - ENSP00000384529 [100%]
NASP-202 - ENSP00000438871 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:400 1:1000 1:2500 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly not consistent with RNAseq data Mainly not consistent with RNAseq data Mainly consistent with RNAseq data 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:50 1:26 1:161 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400884)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400884)
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

86.3, 85.2, 48.8, 28.2, 16.7 86.3, 85.2, 78.4, 48.8, 45.8 86.3, 85.2, 78.4, 34.3 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         siRNA

Image

   

Description

Application not done for this antibody. Signal downregulation > 25% by one siRNA.
More information
 Application not done for this antibody. 

Antibody dilution

 1:26  

Validation siRNA

 Supportive: The siRNA validation is supportive.  
 

ANTIGEN VIEW

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NASP-001
 
NASP-004
 
NASP-005
 
NASP-007
 
NASP-009
 
NASP-015
 
NASP-201
 
NASP-202