NCKAP5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA034639

 

Antibody HPA044859

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA034639 HPA044859 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

81 94 

Antigen sequence

SKLTHSVSDSLFGWETNRKHFLEGTSSVYPKERPEKLTSCASSCPLEMKL
CPSVQTPQVQRERGPQGQGHGRMALNLQLSD
 
PELQCETENELIKDTKSADNPDGGLQSKNNRRTPQDIYNQLKIEPRNRHS
PVACSTKDTFMTELLNRVDKKAAPQTESGSSNASCRNVLKGSSQ
 

Matching transcripts

NCKAP5-001 - ENSP00000387128 [100%]
NCKAP5-201 - ENSP00000380603 [100%]
 NCKAP5-001 - ENSP00000387128 [100%]
NCKAP5-201 - ENSP00000380603 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:10  

Literature conformity

Partly consistent with gene/protein characterization data  

RNA consistency

Not at all consistent with RNAseq data  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

208.5 208.5 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:6000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line HeLa shows positivity in nucleoli & the Golgi apparatus.
More information
 

Antibody dilution

 1:145 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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NCKAP5-001
 
NCKAP5-201