VEPH1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026645

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026645 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

134 

Antigen sequence

RAGDLFSLDDSEIEDSLTEALEQIKIISSSSDYQTNNNDQAVVEICITRI
TTAIRETESIEKHAKALVGLWDSCLEHNLRPFGKDEDTPHAKIASDIMSC
ILQNYNRPPVMALAIPIAVKFLHRGNKELCRNMS
 

Matching transcripts

VEPH1-001 - ENSP00000354919 [100%]
VEPH1-002 - ENSP00000417268 [100%]
VEPH1-003 - ENSP00000419193 [100%]
VEPH1-004 - ENSP00000376578 [100%]
VEPH1-005 - ENSP00000376577 [100%]
VEPH1-011 - ENSP00000420097 [100%]
VEPH1-201 - ENSP00000443868 [100%]
VEPH1-202 - ENSP00000446258 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in reaction center cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:8 

Literature conformity

Partly consistent with extensive gene/protein characterization data 

RNA consistency

Mainly not consistent with RNAseq data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli & cytoplasm.
More information
 

Antibody dilution

1:20 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403012)
More information
 

Target mass (kDa)

94.7, 89.7, 23.9, 19.8, 18.4 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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VEPH1-001
 
VEPH1-002
 
VEPH1-003
 
VEPH1-004
 
VEPH1-005
 
VEPH1-011
 
VEPH1-201
 
VEPH1-202