SYT15

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA036971

 

Antibody HPA036972

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA036971 HPA036972 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

88 84 

Antigen sequence

KVSLMNHNKFVKCKKTSAVLGSINPVYNETFSFKADATELDTASLSLTVV
QNMEGDKSQQLGRVVVGPYMYTRGRELEHWDEMLSKPK
 
HARTQLSRPPAVPFVVPPTLQGRDWVPLHSGEWADAPWDPCPASELLPHT
SSGGLGDACMVGAINPELYKFPEDKSETDFPDGC
 

Matching transcripts

SYT15-002 - ENSP00000363443 [100%]
SYT15-004 - ENSP00000363441 [100%]
 SYT15-003 - ENSP00000427607 [100%]
SYT15-004 - ENSP00000363441 [100%]
SYT15-201 - ENSP00000363445 [100%]
SYT15-002 - ENSP00000363443 [90%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
More information
 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:100 1:900 

Literature conformity

Not consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Not at all consistent with RNAseq data Not at all consistent with RNAseq data 

         Western Blot

Image

  

Description

  

Target mass (kDa)

52.2, 47.4 52.2, 47.4, 43.5 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:6000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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SYT15-002
 
SYT15-003
 
SYT15-004
 
SYT15-201