The subcellular protein atlas displays high resolution, multicolor images of immunofluorescently stained cells. This provides spatial information on protein expression patterns on a fine cellular and subcellular level. For each antibody two human suitable cell lines from a cell line panel are selected for the immunofluorescence analysis, based on RNA sequencing data. U-2 OS is also always used, making up the third cell line. To validate the results, the corresponding target protein may be downregulated by siRNA prior to immunostaining.

Example proteins in the subcell atlas:
Protein localized to the plasma membrane in both human and mouse cells.
Zinc finger protein-like 1.
Protein localized to the Golgi apparatus.
Heterogeneous nuclear ribonucleoprotein U-like 1.
Localized to the nucleus but excluded from the nucleoli in human and mouse cells. The location has been validated by siRNA mediated gene silencing.


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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.