SPEN

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA015825

 

Antibody HPA050257

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA015825 HPA050257 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

146 134 

Antigen sequence

NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRS
RSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQ
HSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI
 
GEAVENQEVQSKKPIPSKPQLKQLQVLDDQGPEREDVRKNYCSLRDETPE
RKSGQEKSHSVNTEEKIGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKF
GSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSK
 

Matching transcripts

SPEN-001 - ENSP00000364912 [100%]
SPEN-002 - ENSP00000388021 [100%]
 SPEN-001 - ENSP00000364912 [99%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human rectum shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:450  

Literature conformity

Consistent with extensive gene/protein characterization data  

RNA consistency

Consistent with RNAseq data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:143 1:43 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

 Target too small/large to be analyzed with the present setup 

Target mass (kDa)

402.2, 43 402.2 

Antibody dilution

1:250  

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Target too small/large to be analyzed with the present setup 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:1700 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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SPEN-001
 
SPEN-002