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Antibody HPA039247
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Antibody HPA043684
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Antibody HPA063394
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Antibody CAB004621
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Antibody CAB005887
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ANTIBODY INFORMATION
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Provider |
Atlas Antibodies Sigma-Aldrich
| | Atlas Antibodies Sigma-Aldrich
| | Atlas Antibodies Sigma-Aldrich
| | Santa Cruz Biotechnology
| | Abcam plc
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Product name |
HPA039247 | | HPA043684 | | HPA063394 | | sc-5286 | | ab4074 | |
Host species |
Rabbit | | Rabbit | | Rabbit | | Mouse | | Rabbit | |
Clonality |
pAb | | pAb | | pAb | | mAb | | msAb | |
Purity |
Affinity purified using the PrEST-antigen as affinity ligand | | Affinity purified using the PrEST-antigen as affinity ligand | | Affinity purified using the PrEST-antigen as affinity ligand | | Protein A/G | | Affinity | |
Released in version |
10 | | 10 | | 13 | | 3 | | 3 | |
ANTIGEN INFORMATION
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Antigen |
Recombinant protein fragment | | Recombinant protein fragment | | Recombinant protein fragment | | Recombinant protein | | Synthetic peptide | |
Length (aa) |
28 | | 24 | | 29 | | | | | |
Antigen sequence |
LEHGIQPDGQMPSDKTIGGGDDSFNTFF
| | ATYAPVISAEKAYHEQLSVAEITN
| | NTTAIAEAWARLDHKFDLMYAKRAFVHWY
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Matching transcripts |
TUBA4A-001 - ENSP00000248437 [96%] TUBA4A-002 - ENSP00000375938 [96%] TUBA4A-003 - ENSP00000396061 [96%] TUBA4A-004 - ENSP00000416992 [96%] TUBA4A-009 - ENSP00000404740 [96%] TUBA4A-010 - ENSP00000408194 [96%]
| | TUBA4A-001 - ENSP00000248437 [100%] TUBA4A-002 - ENSP00000375938 [100%] TUBA4A-011 - ENSP00000396212 [100%]
| | TUBA4A-001 - ENSP00000248437 [100%] TUBA4A-002 - ENSP00000375938 [100%]
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Other gene match |
TUBA3D - ENSG00000075886 [100%] TUBA1B - ENSG00000123416 [100%] TUBA3E - ENSG00000152086 [100%] TUBA1A - ENSG00000167552 [100%] TUBA1C - ENSG00000167553 [100%] TUBA3C - ENSG00000198033 [100%]
| | TUBA3D - ENSG00000075886 [100%] TUBA1B - ENSG00000123416 [100%] TUBA3E - ENSG00000152086 [100%] TUBA1A - ENSG00000167552 [100%] TUBA1C - ENSG00000167553 [96%] TUBA8 - ENSG00000183785 [88%] TUBA3C - ENSG00000198033 [100%]
| | TUBA3D - ENSG00000075886 [100%] TUBA1B - ENSG00000123416 [100%] TUBA3E - ENSG00000152086 [93%] TUBA1A - ENSG00000167552 [100%] TUBA1C - ENSG00000167553 [97%] TUBAL3 - ENSG00000178462 [93%] TUBA8 - ENSG00000183785 [100%] TUBA3C - ENSG00000198033 [100%]
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ANTIBODY VALIDATION
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Immunohistochemistry
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Image |
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Description |
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts. More information | | Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of the seminiferous ducts. More information | | Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts. More information | | Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. More information | | Immunohistochemical staining of human small intestine shows distinct cytoplasmic positivity in glandular cells. More information | |
Retrieval method |
HIER pH6 | | HIER pH6 | | HIER pH6 | | HIER pH6 | | HIER pH6 | |
Antibody dilution |
1:600 | | 1:150 | | 1:40 | | 1:200 | | 1:25 | |
Literature conformity |
Partly consistent with extensive gene/protein characterization data | | Consistent with extensive gene/protein characterization data | | Consistent with gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | | Partly consistent with extensive gene/protein characterization data | |
RNA consistency |
Mainly consistent with RNAseq data | | Not done | | Mainly consistent with RNAseq data | | Not done | | Not done | |
Western Blot
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Description |
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | | Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil More information | |
Target mass (kDa) |
57.7, 50.2, 50.1, 50, 49.9, 48.3, 27.6, 24.8, 21.7, 19.8, 16.9, 14.4, 13.2 | | 57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 43, 31, 17.7 | | 57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 45.5, 43, 31 | | 49.9, 48.3, 21.7, 19.8, 17.7, 16.9, 13.2 | | 49.9, 48.3, 21.7, 19.8, 17.7, 16.9, 13.2 | |
Antibody dilution |
1:250 | | 1:250 | | 1:80 | | 1:500 | | 1:500 | |
Validation WB |
Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | | Supportive: Single band corresponding to the predicted size in kDa (+/-20%) | |
Protein array
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Description |
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green. More information | | Application not done for this antibody. | | Application not done for this antibody. | |
Antibody dilution |
1:500 | | 1:3000 | | 1:500 | | | | | |
Validation PA |
Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | Supportive: Pass with single peak corresponding to interaction only with its own antigen. | | | | | |
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