HIST3H3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042570

 

Antibody CAB001386

 

Antibody CAB003812

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Upstate
 Epitomics
 

Product name

HPA042570 06-599 1326-1 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known Supernatant 

Released in version

9 1 2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known Synthetic peptide 

Length (aa)

25   

Antigen sequence

SEAYLVGLFEDTNLCAIHAKRVTIM
 
 
 

Matching transcripts

HIST3H3-001 - ENSP00000355657 [88%]
   

Other gene match

HIST1H3B - ENSG00000124693 [96%]
H3F3B - ENSG00000132475 [100%]
H3F3A - ENSG00000163041 [100%]
HIST1H3I - ENSG00000182572 [96%]
HIST2H3D - ENSG00000183598 [100%]
H3F3C - ENSG00000188375 [96%]
HIST1H3C - ENSG00000196532 [96%]
HIST1H3E - ENSG00000196966 [96%]
HIST1H3J - ENSG00000197153 [96%]
HIST1H3D - ENSG00000197409 [96%]
HIST1H3A - ENSG00000198366 [96%]
HIST2H3C - ENSG00000203811 [100%]
HIST1H3H - ENSG00000203813 [96%]
HIST2H3PS2 - ENSG00000203818 [96%]
HIST2H3A - ENSG00000203852 [100%]
HIST1H3G - ENSG00000256018 [96%]
HIST1H3F - ENSG00000256316 [96%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human vulva/anal skin shows strong nuclear positivity in epidermal cells.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:30 1:1000 1:200 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done Not done 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

15.5, 15.4, 15.3, 15.2, 14.9 15.5 15.5 

Antibody dilution

1:250 1:500 1:500 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

  1:50 

Validation IF

  Supportive: The subcellular location is supported by literature. 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:3000   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

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HIST3H3-001