HIST1H3G

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042570

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042570 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

9 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

25 

Antigen sequence

SEAYLVGLFEDTNLCAIHAKRVTIM
 

Matching transcripts

HIST1H3G-001 - ENSP00000439660 [96%]
 

Other gene match

HIST1H3B - ENSG00000124693 [96%]
H3F3B - ENSG00000132475 [100%]
H3F3A - ENSG00000163041 [100%]
HIST3H3 - ENSG00000168148 [88%]
HIST1H3I - ENSG00000182572 [96%]
HIST2H3D - ENSG00000183598 [100%]
H3F3C - ENSG00000188375 [96%]
HIST1H3C - ENSG00000196532 [96%]
HIST1H3E - ENSG00000196966 [96%]
HIST1H3J - ENSG00000197153 [96%]
HIST1H3D - ENSG00000197409 [96%]
HIST1H3A - ENSG00000198366 [96%]
HIST2H3C - ENSG00000203811 [100%]
HIST1H3H - ENSG00000203813 [96%]
HIST2H3PS2 - ENSG00000203818 [96%]
HIST2H3A - ENSG00000203852 [100%]
HIST1H3F - ENSG00000256316 [96%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human vulva/anal skin shows strong nuclear positivity in epidermal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:30 

Literature conformity

Consistent with extensive gene/protein characterization data 

RNA consistency

Not done 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

15.5, 15.4, 15.3, 15.2, 14.9 

Antibody dilution

1:250 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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HIST1H3G-001