CSNK2B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA005944

 

Antibody CAB004349

 

Antibody CAB013087

 

Antibody CAB016059

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 BioSource
 Santa Cruz Biotechnology
 

Product name

HPA005944 sc-46666 44-1090G sc-12739 

Host species

Rabbit Mouse Rabbit Mouse 

Clonality

pAb mAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G Not known Protein A/G 

Released in version

4 3 4 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein Not known Not known 

Length (aa)

145    

Antigen sequence

PHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRG
IAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDV
YTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLY
 
 
 
 

Matching transcripts

CSNK2B-001 - ENSP00000365042 [100%]
CSNK2B-004 - ENSP00000365046 [100%]
CSNK2B-008 - ENSP00000365025 [100%]
CSNK2B-009 - ENSP00000365026 [100%]
    

Other gene match

CSNK2B-LY6G5B-1181 - ENSG00000263020 [98%]
    

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
More information
 Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
More information
 Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
More information
 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic as well as nuclear positivity in neuronal cells and glial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:75 1:300 1:500 1:75 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done Not done Not done 

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

26.9, 26.7, 24.9 26.9, 24.9 26.9, 24.9 26.9, 24.9 

Antibody dilution

1:250 1:500 1:500 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:500    

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).    

         Immunofluorescence

Image

    

Description

Application not done for this antibody. Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

  1:125  

Validation IF

  Uncertain: The subcellular location is partly supported by literature or no literature is available.  
 

ANTIGEN VIEW

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CSNK2B-001
 
CSNK2B-004
 
CSNK2B-008
 
CSNK2B-009