CSNK2B-LY6G5B-1181

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA005944

 

Antibody HPA048307

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA005944 HPA048307 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

145 95 

Antigen sequence

PHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRG
IAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDV
YTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLY
 
CCQYDYCNSWSSPQLQSSLPEPHDRPLALPLSDSQIQWFYQALNLSLPLP
NFHAGTEPDGLDPMVTLSLNLGLSFAELRRMYLFLNSSGLLVLPQ
 

Matching transcripts

CSNK2B-LY6G5B-1181-001 - ENSP00000365040 [98%]
 CSNK2B-LY6G5B-1181-002 - ENSP00000387322 [100%]
 

Other gene match

CSNK2B - ENSG00000204435 [100%]
 LY6G5B - ENSG00000240053 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
More information
 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non-germinal center cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:75 1:100 

Literature conformity

Partly consistent with extensive gene/protein characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

26.9, 26.7, 24.9 23.9, 22.6, 16.8 

Antibody dilution

1:250 1:90 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:700 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
CSNK2B-LY6G5B-1181-001
 
CSNK2B-LY6G5B-1181-002