DEPDC5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA054969

 

Antibody HPA055619

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA054969 HPA055619 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

87 87 

Antigen sequence

GIGVDLVCVGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTS
KSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPK
 
YDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTL
PTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSS
 

Matching transcripts

DEPDC5-001 - ENSP00000383107 [99%]
DEPDC5-002 - ENSP00000383101 [99%]
DEPDC5-004 - ENSP00000371545 [99%]
DEPDC5-006 - ENSP00000371546 [99%]
DEPDC5-201 - ENSP00000266091 [99%]
DEPDC5-202 - ENSP00000371539 [99%]
DEPDC5-203 - ENSP00000383105 [99%]
DEPDC5-204 - ENSP00000383108 [99%]
DEPDC5-205 - ENSP00000440210 [99%]
DEPDC5-206 - ENSP00000441358 [99%]
 DEPDC5-001 - ENSP00000383107 [100%]
DEPDC5-002 - ENSP00000383101 [100%]
DEPDC5-004 - ENSP00000371545 [100%]
DEPDC5-006 - ENSP00000371546 [100%]
DEPDC5-201 - ENSP00000266091 [100%]
DEPDC5-202 - ENSP00000371539 [100%]
DEPDC5-203 - ENSP00000383105 [100%]
DEPDC5-204 - ENSP00000383108 [100%]
DEPDC5-205 - ENSP00000440210 [100%]
DEPDC5-206 - ENSP00000441358 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.
More information
 Immunohistochemical staining of human stomach, upper shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:100 1:200 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Mainly not consistent with RNAseq data Mainly not consistent with RNAseq data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

180.4, 178.8, 177.9, 173.4, 170.5, 138.3, 75.4, 63.7 180.4, 178.8, 177.9, 173.4, 170.5, 138.3, 75.4, 63.7 

Antibody dilution

1:100 1:750 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:850 1:6050 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line HeLa shows positivity in the Golgi apparatus.
More information
 

Antibody dilution

 1:152 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

? »
 
 
 
DEPDC5-001
 
DEPDC5-002
 
DEPDC5-004
 
DEPDC5-006
 
DEPDC5-201
 
DEPDC5-202
 
DEPDC5-203
 
DEPDC5-204
 
DEPDC5-205
 
DEPDC5-206