SLC39A8

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038832

 

Antibody HPA038833

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038832 HPA038833 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

73 88 

Antigen sequence

LKTYGQNGHTHFGNDNFGPQEKTHQPKALPAINGVTCYANPAVTEANGHI
HFDNVSVVSLQDGKKEPSSCTCL
 
ANLSLSAAQLQHLLEQMGAASRVGVPEPGQLHFNQCLTAEEIFSLHGFSN
ATQITSSKFSVICPAVLQQLNFHPCEDRPKHKTRPSHS
 

Matching transcripts

SLC39A8-001 - ENSP00000349174 [100%]
SLC39A8-002 - ENSP00000378310 [100%]
SLC39A8-003 - ENSP00000394548 [100%]
 SLC39A8-001 - ENSP00000349174 [100%]
SLC39A8-002 - ENSP00000378310 [100%]
SLC39A8-003 - ENSP00000394548 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human placenta shows distinct membranous positivity in syncytiotrophoblasts.
More information
 Immunohistochemical staining of human placenta shows distinct membranous positivity in syncytiotrophoblasts.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:10 1:30 

Literature conformity

Consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

49.6, 48.1 49.6, 48.1 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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SLC39A8-001
 
SLC39A8-002
 
SLC39A8-003